Peroxidase Gene Nematode Inducible Promotors and Methods of Use
AssigneeBASF Plant Science GmbH
InventorsAaron Wiig, Robert Ascenzi, Xiang Huang, Sumita Chaudhuri, Rui-Guang Zhen, Yu Han
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Abstract
The invention provides plant gene promoters and regulatory elements that are root-specific and/or induced by parasitic nematodes. The promoters of the invention are useful for controlling expression of nucleic acids of interest in plants roots.
Claims21
A promoter comprising an isolated polynucleotide capable of mediating root-preferred or nematode-inducible expression, said polynucleotide being selected from the group consisting of:
- a) a polynucleotide having the sequence as set forth in SEQ ID NO:1;
- b) a polynucleotide comprising nucleotides 1677 to 2027 or nucleotides 1604 to 2085 of a polynucleotide having the sequence as set forth in SEQ ID NO:1;
- c) a polynucleotide having at least 70% sequence identity to the polynucleotide of a) or b);
- d) a polynucleotide hybridizing under stringent conditions to the polynucleotide of a) or b);
- e) a polynucleotide comprising a biologically active portion of the polynucleotide of a) or b);
- and
- f) a polynucleotide comprising a fragment of at least 50 consecutive nucleotides, or at least 100 consecutive nucleotides, or at least 200 consecutive nucleotides of a polynucleotide having the sequence as set forth in SEQ ID NO:1.
A transgenic plant transformed with an expression cassette, wherein the expression cassette comprises an isolated promoter polynucleotide capable of mediating root-preferred or nematode-inducible expression, said promoter polynucleotide being selected from the group consisting of:
- a) a polynucleotide having the sequence as set forth in SEQ ID NO:1;
- b) a polynucleotide comprising nucleotides 1677 to 2027 or nucleotides 1604 to 2085 of a polynucleotide having the sequence as set forth in SEQ ID NO:1;
- c) a polynucleotide having at least 70% sequence identity to the polynucleotide of a) or b);
- d) a polynucleotide hybridizing under stringent conditions to the polynucleotide of a) or b);
- e) a polynucleotide comprising a biologically active portion of the polynucleotide of a) or b);
- and
- f) a polynucleotide comprising a fragment of at least 50 consecutive nucleotides, or at least 100 consecutive nucleotides, or at least 200 consecutive nucleotides of a polynucleotide having the sequence as set forth in SEQ ID NO:1.
Dependencies are inferred from the claim text.