Transferases, Epimerases, Polynucleotides Encoding These and Uses Thereof
AssigneeThe New Zealand Institute for Plant and Food Research Limited
InventorsWilliam A. Laing, Sean M.W. Bulley
Life of this patent
Estimating the term…
Abstract
The invention provides compositions and methods for modulating GDP-L-Galactose Guanyltransferase (also known as GDP-L-Galactose phosphorylase) activity; and/or GDP-D-Mannose epimerase activity; and/or ascorbate content in plants. The invention provides plants and plant cells with increased GDP-L-Galactose Guanyltransferase activity; and/or GDP-D-Mannose epimerase activity. The invention provides plants and plant cells with increased ascorbate content as a result of: over-expression of GDP-L-Galactose Guanyltransferase; over-expression of GDP-D-Mannose epimerase; or in particular over-expression of a combination of GDP-L-Galactose Guanyltransferase and GDP-D-Mannose epimerase.
Claims32
1-62. (canceled)
A method for producing a plant cell or plant with increased ascorbate, the method comprising transformation of a plant cell or plant with a polynucleotide encoding a polypeptide with the amino acid sequence of any one of SEQ ID NO:1 to 11, or a variant of the polypeptide, wherein the variant has the activity of an GDP-L-Galactose Guanyltransferase.
A method for producing a plant cell or plant with increased ascorbate content, the method comprising transformation of a plant cell or plant with:
- a) a polynucleotide encoding a GDP-D-Mannose epimerase;
- and
- b) a polynucleotide encoding a GDP-L-Galactose Guanyltransferase.
The method of claim 697 in which the GDP-D-Mannose epimerase comprises the amino acid sequence AADMGGMGFIQSNHSVI (SEQ ID NO:
- 36).
An isolated polynucleotide encoding a polypeptide comprising a sequence with at least 78% identity to the sequence of any one of SEQ ID NO:
- 1 to 7, wherein the polypeptide is a GDP-L-Galactose Guanyltransferase.
Dependencies are inferred from the claim text.