Screening for vase-life and for resistance to xanthomonas in anthurium
AssigneeUniversity of the West Indies, a Regional Instutio established by Royal Charter
InventorsPathmanathan Umaharan, Winston Elibox
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Abstract
Anthurium species are important ornamental crops that are susceptible to infection by Xanthomonas campestris pv. Dieffenbachiae. Methods of identifying target plants that are resistant to or tolerant of Xanthomonas infection are described. Methods of predicting vase-life performance are also disclosed.
Claims15
A method of identifying resistance or tolerance of a plant to Xanthomonas infection, the method comprising
- a. providing a target plant;
- b. infecting the target plant with a fluorescent Xanthomonas expressing a heterologous fluorescent protein;
- and
- c. detecting the level of fluorescent protein in the target plant, wherein a level of fluorescent protein in the target plant that is less than the level in a susceptible target plant indicates that the target plant is resistant to or tolerant of infection by the Xanthomonas.
The method of claim 10, further comprising detecting foliar resistance.
A method of identifying resistance or tolerance of a plant to Xanthomonas infection, the method comprising
- a. providing a leaf sample from a target plant;
- b. infecting the leaf sample with a Xanthomonas;
- c. detecting Xanthomonas infection in the infected leaf sample;
- and
- d. comparing the rate of infection to a reference to determine foliar resistance of the target plant.
A recombinant X. campestris comprising a fluorescent protein.
A method of predicting vase-life performance of an anthurium, the method comprising
- a. providing a sample from an anthurium;
- b. determining abaxial stomata number and spathe color in the anthurium sample;
- and
- c. comparing abaxial stomata number and spathe color to a control or reference set, thereby predicting vase-life performance of the anthurium.
A method of predicting vase-life performance of an anthurium, the method comprising
- a. providing an anthurium;
- b. determining the average time for spadix necrosis of the anthurium;
- and
- c. comparing the average time for spadix necrosis of the anthurium to a reference, thereby predicting the vase-life performance of the anthurium.
Dependencies are inferred from the claim text.