Cybrid Plant of the Genus Lactuca and Method for Producing the Same
AssigneeSakata Seed Corporation
InventorShingo Horiuchi
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Abstract
The object of the present invention is the provision of a cybrid plant of the genus Lactuca which is useful for producing a first filial generation seed, and a method for producing the same. The present invention is directed to a cybrid plant of the genus Lactuca, a progeny thereof, or a part thereof, comprising, in cytoplasm thereof, a gene derived from mitochondria of a plant of the genus Helianthus. The cybrid plant is preferably cytoplasmic male sterile. The present invention is also directed to a method for producing the cybrid plant of the genus Lactuca.
Claims24
1-94. (canceled)
A cybrid plant of the genus Lactuca, a progeny thereof, or a part thereof, comprising, in cytoplasm thereof, a gene derived from mitochondria of a plant of the genus Helianthus and being cytoplasmic male sterile.
A seed of the cybrid plant of the genus Lactuca that has been deposited under Accession No. FERM BP-10421, a cybrid plant of the genus Lactuca grown from the seed, or the progeny thereof, or a part thereof.
A seed of the cybrid plant of the genus Lactuca that has been deposited under Accession No. FERM BP-10647, a cybrid plant of the genus Lactuca grown from the seed, or the progeny thereof, or a part thereof.
A method for producing a cybrid plant of the genus Lactuca comprising, in cytoplasm thereof, a gene derived from mitochondria of a plant of the genus Helianthus and being cytoplasmic male sterile, characterized by comprising:
- fusing protoplasts from a plant of the genus Helianthus with protoplasts from a plant of the genus Lactuca;
- culturing one or more of the fused cells;
- and regenerating a plant of the genus Lactuca from cells cultured from one or more of the fused cells, and wherein the step of culturing the fused cells comprises culturing the fused cells in a liquid medium;
- and then adding gellan gum to the medium to be semisolid and continuing the culturing.
Dependencies are inferred from the claim text.