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US 2010/0076380 A1Published application
Bioreactors
AssigneesAmprotein Corporation, Hangzhou AmProtein Bioengineering Co., Ltd.
InventorMizhou Hui
Life of this patent
Estimating the term…
Abstract
Disclosed are various examples of methods and systems for providing an oxygenated culture environment for growing animal cells, as well as methods and systems for oxygenating blood and related methods for treating diseases and related conditions.
Claims54
A cell culture apparatus comprising:
- a rotatable vessel having:
- a wall for defining a culture chamber, the wall having an inner surface configured to promote gas-inner surface contact;
- and
- an inlet formed in the wall enabling fluid communication between the culture chamber and an external fluid source;
- and
- an actuator coupled to the vessel for rotating the vessel about a first axis to increase the level of dissolved oxygen in a fluid in the culture chamber.
3 more elements
A method for oxygenating a culture medium comprising:
- introducing a culture medium into a culture chamber at least partially filled with gas, wherein the culture chamber is defined by a wall of a vessel, and an inner surface of the wall is configured to promote gas-inner surface contact;
- and
- rotating the vessel to create a relative movement of the culture medium with respect to the inner surface of the wall of the vessel to increase the level of dissolved oxygen in the culture medium.
A culture system comprising:
- an oxygenation apparatus configured for oxygenating a culture medium, the oxygenation apparatus including:
- a vessel having an inlet for receiving the culture medium to be oxygenated, an outlet for providing oxygenated culture medium, and a wall with an inner surface configured to promote gas-inner surface contact;
- and
- an actuator coupled to the vessel for driving the vessel in orbital movement to increase the level of dissolved oxygen in the culture medium;
- and
- a culture apparatus configured for receiving the oxygenated culture medium from the oxygenation apparatus, the culture apparatus including:
- a port for receiving a biological material into an interior of the culture apparatus;
- and
- an inlet in fluid communication with the interior of the culture apparatus to deliver the oxygenated culture medium into contact with the biological material.
6 more elements
A culture system comprising:
- a housing having a first terminus and a second and close terminus opposed to the first terminus, the first terminus having a port for receiving and dispensing a culture medium;
- a tubular member disposed within an interior of the housing, the tubular member having:
- a first open end;
- a second and close end positioned at the second terminus of the housing;
- and
- a disk membrane coupled to the first open end for at least partially enclosing an interior of the second tubular member to define a culture chamber, wherein the disk member includes at least an opening enabling fluid communication between the culture chamber and an exterior of the second tubular member;
- and
- an actuator coupled to the housing for rotating the housing to increase the level of dissolved oxygen in a fluid in the culture chamber.
5 more elements
A cell culture apparatus comprising:
- a tubular member having a first end and a second and close end opposed to the first end, the first end having a port for receiving and dispensing a culture medium;
- a bio-compatible matrix disposed within an interior of the tubular member;
- and
- a disk member detachably disposed within the interior of the tubular member for substantially confining the bio-compatible matrix within a culture chamber defined between the disk member and the second end of the tubular member;
- wherein the disk member includes at least an opening for permitting a flow of the culture medium into the culture chamber.
2 more elements
A cell culture system comprising:
- an elongated member having:
- a first close end;
- a second close end opposed to the first close end;
- a wall defining a culture chamber at least partially filled with gas;
- and
- an inlet formed in the wall capable of receiving a culture medium;
- a shaft positioned substantially within the culture chamber, the shaft having a first end and a second end respectively positioned at a corresponding end of the elongated member;
- a set of one or more disk elements concentrically mounted on the shaft;
- an actuator mechanically coupled to the shaft for rotating the disk elements about a longitudinal axis of the shaft for inducing a relative movement of the culture medium with respect to an inner surface of the wall of the elongated member;
- and
- a sensor for detecting the level of dissolved oxygen in the culture medium contained in the culture chamber.
8 more elements
A system for oxygenating blood and delivering the oxygenated blood to a subject, the system comprising:
- (1) a blood pump assembly adapted for coupling to a supply of blood;
- (2) a blood oxygenation assembly coupled to the blood pump assembly, the blood oxygenation assembly receiving the blood from the blood pump assembly and oxygenating the blood, the blood oxygenation assembly comprising a blood bioreactor/oxygenator, wherein the blood bioreactor/oxygenator comprises:
- (a) a housing including a wall;
- (b) a first inlet in the housing adapted to deliver oxygen-containing gas stream into contact with a first surface of the wall;
- (c) a second inlet in the housing adapted to deliver the blood into contact with a second surface of the wall, wherein the blood bioreactor/oxygenator allows a movement of the blood so that the blood contacts with the first surface to form oxygenated blood;
- and
- (d) a first outlet and a second outlet in the housing for expelling CO2-containing gas and the oxygenated blood, respectively;
- (3) a delivery assembly coupled to the second outlet, the delivery assembly being adapted to deliver the oxygenated blood to the subject;
- and
- (4) a control assembly coupled to the blood oxygenation assembly.
7 more elements
Dependencies are inferred from the claim text.