Selective Processing of Biological Material on a Microarray Substrate
OwnerNils Adey, Arnold Oliphant +1inventors
InventorsNils Adey, Arnold Oliphant, Wanyuan Ao
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Abstract
The present invention provides methods and systems for selectively eluting biological material from a distinct spatial location on a microarray slide. In one aspect, for example, a method for recovering biological material coupled to a microarray slide can include selecting a biological material to be recovered from the microarray slide, finding the biological material within a distinct spatial region on the microarray slide surface, and eluting at least a portion of the selected biological material from the distinct spatial region without eluting substantial amounts of non-selected biological material from regions of the microarray slide that are not within the distinct spatial region.
Claims29
A method for recovering biological material coupled to a microarray slide, comprising:
- selecting a biological material to be recovered from the microarray slide;
- finding the biological material within a distinct spatial region on the microarray slide surface;
- and
- eluting at least a portion of the selected biological material from the distinct spatial region without eluting substantial amounts of non-selected biological material from regions of the microarray slide that are not within the distinct spatial region.
A method for recovering nucleic acid material from a microarray, comprising:
- selecting a nucleic acid material that has been hybridized onto a microarray slide surface in a distinct spatial region;
- applying a denaturing buffer to the distinct spatial region to at least partially denature the selected nucleic acid material;
- and
- flushing the microarray surface with an inert recovery buffer to recover the denatured portion of the selected nucleic acid material.
A system for recovering nucleic acid material from a microarray, comprising:
- a microarray scanner configured to scan a microarray surface and identify a location of a nucleic acid material to be recovered;
- a dispensing instrument configured to receive input from the microarray scanner and dispense an elution buffer on a discrete dispensing area of the microarray surface at the location indicated by the microarray scanner;
- and
- a recovery instrument configured to recover the elution buffer from the microarray surface.
A method for selectively labeling biological material coupled to a microarray slide, comprising:
- selecting a biological material to be labeled;
- locating the biological material within a distinct spatial region on a surface of the microarray slide;
- and
- labeling at least a portion of the selected biological material from the distinct spatial region without labeling substantial amounts of non-selected biological material from regions of the microarray slide that are not within the distinct spatial region.
A method for selectively amplifying biological material coupled to a microarray slide, comprising:
- selecting a biological material to be amplified;
- locating the biological material within a distinct spatial region on the microarray slide surface;
- and
- amplifying at least a portion of the selected biological material from the distinct spatial region without amplifying substantial amounts of non-selected biological material from regions of the microarray slide that are not within the distinct spatial region.
Dependencies are inferred from the claim text.