Sweetener Preparations and Methods of Use
AssigneeLoren Miles
InventorsLoren Miles, Michael Louie, Ven Kiteswaran Subramanian
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Abstract
The present disclosure relates to codon-optimized brazzein coding sequences and the expression of brazzein and variants thereof using yeast expression systems. The disclosure also relates to methods of expression of proteins to enhance the sweetness taste profile of foods and/or beverages.
Claims17
A method for preparing a brazzein protein, comprising:
- expressing in a yeast host cell an expression vector comprising a promoter operably linked to a nucleic acid molecule encoding a brazzein polypeptide.
An isolated nucleic acid molecule comprising the codon optimized sequence homolog of SEQ ID NOS:
- 1-2 suitable for expression in Pichia pastoris or Saccharomyces cerevisiae.
A yeast expression vector comprising a nucleic acid molecule further comprising SEQ ID NOS:
- 1-2.
An isolated recombinant yeast host cell comprising at least one nucleic acid molecule of SEQ ID NOS:
- 7-19.
Dependencies are inferred from the claim text.