Rapid immunochromatographic detection by amplification of the colloidal gold signal
AssigneesThe Jordanian Pharmaceutical Manufacturing Co., Terramark Markencreation GmbH
InventorsAdnan Badwan, Murshed Abdel-Qader Mohammed
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Abstract
The present invention relates to a method for rapid immunochromatographic detection of a target in a sample, wherein the target is an antibody and/or an antigen, using different colloidal gold conjugates conjugated with a specific antibody and some oligonucleotides and their complementary oligonucleotides and/or antibodies and their related antigens, rapid immunochromatographic detection devices, uses of the method for detecting diseases or specific conditions, and a method for the manufacture of the devices as well as a kit which comprises the devices.
Claims36
A method for rapid immunochromatographic detection of a target in a sample comprising the step of forming a sandwich, by contacting
- (i) a first colloidal gold conjugate conjugated with a specific antibody or antigen and at least one oligonucleotide or non-specific antibody or antigen, and
- (ii) a second colloidal gold conjugate conjugated with at least one complementary oligonucleotide or non-specific antibody or antigen.
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Dependencies are inferred from the claim text.