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US 2010/0075424 A1Published application
Methods and compositions for genetically engineering clostridia species
AssigneeNorthwestern University
InventorsBryan P. Tracy, Eleftherios T. Papoutsakis
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Abstract
The present invention relates to methods and compositions for engineering Clostridia species. In particular, embodiments of the present invention relate to the expression of recombinant resolvase proteins in Clostridia species.
Claims22
A method for incorporating genetic material into a bacterial genome, wherein said bacterial genome lacks a functional resolvase gene, comprising:
- contacting a bacterial cell comprising a bacterial genome with at least one plasmid comprising a gene encoding a resolvase protein and a nucleic acid of interest under conditions such that said nucleic acid of interest integrates into said bacterial genome.
The method of claim 13, wherein said selectable marker is an antibiotic resistance gene.
A method, comprising:
- contacting a bacterial cell comprising a bacterial genome lacking a native resolvase gene with a nucleic acid encoding an exogenous resolvase gene under conditions such that said exogenous resolvase gene is stability incorporated into said bacterial cell.
A bacterial cell comprising an exogenous nucleic acid encoding a resolvase protein.
Dependencies are inferred from the claim text.