Transfection microarrays
AssigneeQiagen GmbH
InventorsWolfgang Bielke, Jie Kang, Karsten Reihs
Life of this patent
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Abstract
A transfection method of introducing sample molecules into cells is provided, comprising: (a) using a substrate comprising at least one sample spot area which is surrounded by an hydrophobic area; (b) wherein sample molecules are applied to said at least one sample spot area, thereby placing said sample molecules in the discrete location of said sample spot area; (c) applying cells to be transfected onto the substrate and incubation under appropriate conditions for entry of the sample molecules into said cells; (d) whereby at least a portion of said sample molecules are introduced into said cells.
Claims26
A transfection method of introducing sample molecules into cells, comprising:
- (a) using a substrate comprising at least one sample spot area which is surrounded by an hydrophobic area;
- (b) wherein sample molecules are applied to said at least one sample spot area, thereby placing said sample molecules in the discrete location of said sample spot area;
- (c) applying cells to be transfected onto the substrate under appropriate conditions for entry of the sample molecules into said cells;
- (d) whereby at least a portion of said sample molecules are introduced into said cells.
A transfection array for introducing sample molecules into cells, comprising a substrate comprising more than 1 sample spot areas arranged in an array format wherein said more than 1 sample spot areas are surrounded by a hydrophobic area and wherein said sample spot areas carry sample molecules useful for an transfection assay, whereby said sample molecules are located in the discrete location of said sample spot areas.
Dependencies are inferred from the claim text.