Methods and devices for isolating embryonic stem cells
AssigneesProteonomix, Inc., The Johns Hopkins University
InventorsMichael Cohen, Xiangcan Zhan, Michael J. Shamblott
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Abstract
Methods, devices and kits are provided for isolating or enriching multicellular embryonic stem (ES) cell colonies from a mixture of multicellular ES cell colonies and single ES cells present in a cellular suspension, by utilizing a filtration matrix that selectively excludes passage of multicellular ES cell colonies. Isolated or enriched multicellular ES cell colonies can be used for propagating pluripotent ES cells.
Claims22
A method for isolating or enriching multicellular ES cell colonies from a mixture of multicellular ES cell colonies and single ES cells present in a cellular suspension comprising the steps of:
- a. providing a filtration matrix through which single ES cells but not multicellular ES cell colonies can pass from a first side to a second side;
- b. contacting the cellular suspension with the first side of the filtration matrix;
- c. passing the cellular suspension from the first side to the second side of the filtration matrix;
- and
- d. collecting the multicellular ES cell colonies from the first side of the filtration matrix, thereby isolating or enriching multicellular ES cell colonies therefrom.
A device for isolating or enriching multicellular ES cell colonies from a mixture of multicellular ES cell colonies and single ES cells present in a cellular suspension comprising:
- a. a filtration matrix through which single ES cells but not multicellular ES cell colonies can pass transversely from a first side to a second side;
- b. means for passing the cellular suspension at least transversely through the filtration matrix;
- and
- c. means for collecting the isolated or enriched multicellular ES cell colonies from the first side of the filtration matrix.
A kit for isolating or enriching multicellular ES cell colonies from a mixture of multicellular ES cell colonies and single ES cells present in a cellular suspension, the kit comprising a filtration matrix through which single ES cells but not multicellular ES cell colonies can pass transversely from a first side to a second side;
- means for passing the cellular suspension at least transversely through the filtration matrix;
- means for collecting the isolated or enriched multicellular ES cell colonies;
- and instructions for use thereof.
Dependencies are inferred from the claim text.