Method for reducing protease activity in plant hydrolysate
AssigneeSchering Corporation
InventorKimberly M. May
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Abstract
The present invention provides methods for reducing protease activity in plant hydrolysates that are utilized in cell culture media. The method involves a combination of heating and ultrafiltration to remove proteases present in the hydrolysate prior to the addition of the hydrolysate to the cell culture medium.
Claims14
A method for reducing protease activity in a cell culture medium containing a soy hydrolysate, comprising the step of heating the soy hydrosylate at more than one temperature for more than about 5 minutes, wherein the heating is performed prior to the addition of the soy hydrolysate to a cell culture medium.
A method for reducing protease activity in a cell culture medium containing a wheat hydrolysate, comprising the step of heating the wheat hydrolysate for one or more minutes prior to the addition of the wheat hydrolysate to a cell culture medium.
A method for reducing protease activity in a cell culture medium containing a hydrolysate, comprising the step of ultrafiltering the hydrolysate through a low molecular weight membrane, wherein said membrane filters out particles having a molecular weight greater than 5 kDa;
- wherein the ultrafiltering is performed prior to the addition of the hydrolysate to a cell culture medium.
A method for reducing protease activity in a cell culture medium containing a hydrolysate, comprising the step of ultrafiltering the hydrolysate through two or more low molecular weight membranes, wherein at least one of the two or more membranes filters out particles having a molecular weight greater than 10 kDa, and wherein the ultrafiltering is performed prior to the addition of the hydrolysate to a cell culture medium.
Dependencies are inferred from the claim text.