Device for microbiological analysis

A device for the microbiological analysis of a support adapted to contain microorganisms marked by a fluorophore agent, said agent being adapted, on contact with said microorganisms, to absorb light energy with an absorption spectrum having a crest at a predetermined absorption wavelength (λ2) and to release that energy by emitting fluorescent light with an emission spectrum having a crest at a predetermined emission wavelength (λ3) distinct from said absorption wavelength (λ2), said device comprising illuminating means adapted to illuminate said support in order for said marked microorganisms to emit, in response to the excitation light emitted by those illuminating means, light with said emission spectrum;

• wherein the spectrum of the excitation light coming from the illuminating means comprises a crest at a predetermined excitation wavelength (λ1) distinct from said absorption (λ2) and emission (λ3) wavelengths of said fluorophore agent, such that said absorption wavelength (λ2) is situated between said excitation (λ1) and emission (λ3) wavelengths with a predetermined difference between the excitation (λ1) and absorption (λ2) wavelengths, by virtue of which it is made easy to discriminate the light emitted by said fluorophore agent from that coming from the illuminating means.