Mutant delta-5 desaturases and their use in making polyunsaturated fatty acids
AssigneeE.I. du Pont de Nemours and Company
InventorsQuinn Qun Zhu, Dana M. Walters Pollak
Life of this patent
Estimating the term…
Abstract
The present invention relates to mutant Δ5 desaturases, which have the ability to convert dihomo-γ-linolenic acid [DGLA; 20:3 ω-6] to arachidonic acid [ARA; 20:4 ω-6] and/or eicosatetraenoic acid [ETA; 20:4 ω-3] to eicosapentaenoic acid [EPA; 20:5 ω-3] and which possess at least one mutation within the HPGG motif of the cytochrome b5-like domain. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ5 desaturases, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”] using these mutant Δ5 desaturases in oleaginous yeast, are disclosed.
Claims12
A mutant polypeptide having Δ5 desaturase activity comprising an amino acid motif selected from the group consisting of:
- SEQ ID NO:183 (HGGG), SEQ ID NO:184 (HHGG), SEQ ID NO:186 (HCGG), SEQ ID NO:187 (HWGG) and SEQ ID NO:185 (HPGS).
Dependencies are inferred from the claim text.