Method for making polyalkylene oxide modified hemoglobin
AssigneeSangart, Inc.
InventorsKim D. Vandegriff, Ashok S. Malavalli, Gnel M. Mkrtchyan
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Abstract
The present invention is a method for preparing a hemoglobin based oxygen carrier to deliver oxygen to tissue. The method comprises mixing a deoxygenated hemoglobin with a maleimide-activated polyakylene oxide (PAO) to obtain an oxygen carrying product. This product is characterized as having 2 to about 8 PAO moieties covalently bound to a deoxygenated hemoglobin and a p50 less than native stroma-free hemoglobin from the same animal source when measured under the same conditions.
Claims9
A method for preparing a polyalkylene oxide (PAO)-hemoglobin (Hb) conjugate that is useful for delivering oxygen to tissue comprising:
- preparing deoxygenated unthiolated hemoglobin from red blood cells;
- and
- mixing the deoxygenated hemoglobin with a maleimide-activated PAO to obtain a Hb-PAO conjugate having PAO moieties covalently bound to cysteine, lysine and histidine moieties on the surface of the deoxygenated hemoglobin;
- wherein the PAO-Hb conjugate has a p50 less than native stroma-free hemoglobin from the same animal source when measured under the same conditions.
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Dependencies are inferred from the claim text.