Methods for the purification of polypeptide conjugates
AssigneeNovo Nordisk A/S
InventorsShawn Defrees, Kyle Kinealy
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Abstract
The present invention provides processes for the manufacturing of polypeptide conjugates. In particular, the invention provides methods for the purification of polypeptide conjugates, which include at least one polymeric modifying groups, such as a poly(alkylene oxide) moiety. Exemplary poly(alkylene oxide) moieties include poly(ethylene glycol) (PEG) and poly(propylene glycol). In an exemplary process, hydrophobic interaction chromatography (HIC) is used to resolve different glycoforms of glycoPEGylated polypeptides.
Claims28
A method of making a composition comprising a first polypeptide conjugate, said first polypeptide conjugate comprising a first number of poly(alkylene oxide) moieties covalently linked to said first polypeptide, said method comprising:
- (a) contacting a mixture comprising said first polypeptide conjugate and a hydrophobic interaction chromatography (HIC) medium;
- and
- (b) eluting said first polypeptide conjugate from said hydrophobic interaction chromatography medium,
- thereby making said composition comprising said first polypeptide conjugate.
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