Multi-Spectral Imaging Including At Least One Common Stain
AssigneeCambridge Research & Instrumentation, Inc.
InventorClifford C. Hoyt
Life of this patent
Estimating the term…
Abstract
A method including: providing a sample with M components to be labeled, where M>2; labeling the components with N stains, where N<M so that at least two components are labeled with a common stain; obtaining a set of spectral images of the sample; classifying different parts of the sample into respective classes that distinguish the commonly stained components based on the set of spectral images; and determining relative amounts of multiple ones of the M components in different regions of the sample. Related apparatus are also disclosed.
Claims25
A method comprising:
- providing a sample with M components to be labeled, where M>2;
- labeling the components with N stains, where N<M so that at least two components are labeled with a common stain;
- obtaining a set of spectral images of the sample;
- classifying different parts of the sample into respective classes that distinguish the commonly stained components based on the set of spectral images;
- and
- determining relative amounts of multiple ones of the M components in different regions of the sample.
A method comprising:
- providing a set of spectral images of a sample, wherein the sample has M>2 components labeled with N<M stains so that at least two components are labeled with a common stain and the sample is further stained with at least one histological stain;
- classifying different parts of the sample into respective classes that distinguish the commonly stained components based on the set of spectral images;
- and
- determining relative amounts of multiple ones of the M components in different regions of the sample.
A method comprising:
- labeling multiple components in a sample with one or more stains that emit or absorb with a common spectral distribution;
- applying a histological stain to the sample;
- obtaining a set of spectral images of the sample based on at least the stains that label the components and the histological stain;
- and
- distinguishing the components labeled with the stains having the common spectral distribution from one another in different parts of the sample based on one or more of signal strength, shape, spectral features, and textural features in one or more of the spectral images that include contributions from at least the histological stain.
Apparatus comprising:
- a means for obtaining a set of spectral images of a sample, wherein the sample has M>2 components labeled with N<M stains so that at least two components are labeled with a common stain and wherein the sample is further stained with at least one histological stain;
- and
- an electronic processor for analyzing an image stack based on the obtained images and configured to classify different parts of the sample into respective classes that distinguish the commonly stained components based on the set of spectral images.
Dependencies are inferred from the claim text.