Media for recovery of microorganism in the presence of antibiotics
AssigneeMillipore Corporation
InventorKathleen Souza
Life of this patent
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Abstract
The present invention provides a media and a method of using such as media for the bioburden or sterility testing of antibiotics or environmental testing of antibiotic manufacturing areas. The medium contains one or more divalent or trivalent cation constituents, preferably of magnesium, calcium, aluminum and iron that allow for microorganism growth even in the presence of residual antibiotic.
Claims20
A process for determining the existence of a microorganism in the presence of an antibiotic which would otherwise inhibit the growth of the microorganism and the antibiotic is not produced by the microorganism, wherein the antibiotic is selected from fluoroquinolones, tetracyclines and aminoglycosides, comprising the steps of:
- a) providing a sample containing an antibiotic to be tested;
- b) providing a holder;
- c) providing a growth medium selected from the group consisting of gel, agar, and broth, wherein the growth medium includes one or more divalent and trivalent cation constituents of magnesium, calcium, aluminium and iron ranging from about 0.1 M to about 0.5M;
- d) placing the sample in contact with the growth media;
- e) incubating the sample and the growth media in the holder for a pre-selected time at a pre-selected temperature;
- and
- f) viewing the growth media to determine the presence of any microorganisms.
A process for testing the bio-burden of a sample wherein the sample includes a microorganism and an exogenously added antibiotic which would otherwise inhibit the growth of the microorganism, and the antibiotic is not produced by the microorganism, wherein the antibiotic is selected from fluoroquinolones, tetracyclines and aminoglycosides comprising the steps of:
- a) providing a sample containing an antibiotic to be tested;
- b) providing a holder;
- c) providing a growth medium selected from the group consisting of gel, agar, and broth, wherein the growth medium includes one or more divalent and trivalent cation constituents of magnesium, calcium, aluminium and iron ranging from about 0.1 M to about 0.5M;
- d) providing a filter selected from a material consisting of regenerated cellulose, mixed cellulose esters, cellulose acetate, cellulose nitrate, nitrocellulose, PVDF, nylons, polycarbonates, polysulfones, polyethersulfones, polyarylsulfones and polyphenylsulfones, and the filter has a pore size about 0.1 microns to about 1.2 microns;
- e) filtering the sample through the filter;
- f) contacting the filter with the growth media;
- g) incubating the filter and the growth media in the holder for period of time from about 0 to 14 days at a temperature from about 20° C. to about 54° C.;
- and
- h) viewing the growth media to determine the presence of any microorganisms.
Dependencies are inferred from the claim text.