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US 2010/0075360 A1Published application
Uv based cell viability as an indicator of sun protection factor and methods of measurement thereof
OwnerMuhammed Majeed, Susmitha Anand +2inventors
InventorsMuhammed Majeed, Susmitha Anand, Geetha Kanhangad Gangadharan, Subbalakshmi Prakash
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Abstract
Disclosed is a simple method of determining SPF based on the staining pattern of viable cells like mouse fibroblasts and human keratinocytes after exposure to ultraviolet radiations. The method provides results comparable to the standard methods of SPF measurement using human subjects.
Claims6
An in-vitro method for determining sun protection factor, said method comprising the steps of:
- (i) Growing suitable cell cultures for 24 hours to form cell monolayers;
- (ii) Exposing the cell monolayers of step (i) to UV irradiation dosages in presence and absence of sample whose Sun protection factor needs to be measured;
- (iii) Staining the UV irradiated monolayers of step (ii) 48 hours after UV irradiation;
- and
- (iv) Quantitating cell death occurring due to UV exposure in the presence and absence of the sample of step (ii) for the determination of Sun Protection factor.
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Dependencies are inferred from the claim text.