Analyte detection

A method for detecting the presence of an analyte in saliva, comprising:

• a) providing an assay test comprising a reaction site that produces a visibly detectable signal in presence of an analyte, wherein said analyte is ethanol;
• wherein said reaction site comprises a chromogen, and wherein said chromogen is potassium iodide, and wherein said reaction site further comprises an alcohol oxidase enzyme and a peroxidase enzyme;
• b) placing said reaction site into a mouth of a subject under conditions such that saliva from said subject is contacted with said reaction site;
• and
• c) detecting the presence or absence of said visibly detectable signal in said reaction site, wherein said visibly detectable signal comprises a color change that occurs at a saliva ethanol concentration equivalent to a blood alcohol concentration at or above 0.04% and wherein no color change occurs at blood alcohol concentrations significantly under 0.04%.