Rapid elisa processes and related compositions
AssigneeJinsite Science & Technology (Nanjing) Co., Ltd.
InventorsZhuying WANG, Fang Liang Zhang, Tao Bai
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Abstract
The present invention provides improved and rapid detection methods for an antigen such as a chemical compound, a peptide, a nucleic acid, or a protein released from cells or virus particles in situ. The detection time for an antigen can be dramatically reduced relative to conventional technologies. The technology can particularly be used, for example, to modify and reduce the detection time significantly in traditional ELISA, and also Western blot or Dot blot assays. The improved ELISA method is rapid, economical, reproducible, simple and automatable. Also provided are compositions and kits for using the improved ELISA methods for the rapid detection of antigens.
Claims7
-13. (canceled)
A method for performing a rapid cell-based enzyme-linked immunosorbent assay (C-ELISA), comprising the steps of:
- (a) lysing cells in a quick lysis and coating buffer;
- (b) coating a solid phase with cellular proteins released in situ in the quick lysis and coating buffer for about two to twenty minutes;
- (c) blocking the solid phase with a blocking reagent dissolved in a quick blocking buffer for between about two to ten minutes;
- (d) (i) incubating the solid phase with a primary detection agent in solution;
- washing the solid phase to remove any unbound primary detection agent;
- and
- detecting the presence of an agent bound on the solid phase with a detection system that detects the bound primary detection agent;
- or
- (ii) incubating the solid phase with a primary detection agent in solution;
- washing the solid phase to remove any unbound primary detection agent;
- incubating the solid phase with a secondary detection agent in solution;
- washing the solid phase to remove any unbound secondary detection agent;
- and
- detecting the presence of an agent bound on the solid phase with a detection system that detects the bound secondary detection agent;
- or
- (iii) incubating the solid phase simultaneously with a primary detection agent and a secondary detection agent in solution;
- washing the solid phase to remove any unbound primary detection agent and secondary detection agent;
- and
- detecting the presence of an agent bound on the solid phase with a detection system that detects the bound secondary detection agent;
- or
- (iv) incubating the solid phase with a primary detection agent in solution;
- washing the solid phase to remove any unbound primary detection agent;
- incubating the solid phase with a secondary detection agent in solution;
- washing the solid phase to remove any unbound secondary detection agent;
- incubating the solid phase with a tertiary detection agent in solution;
- washing the solid phase to remove any unbound tertiary detection agent;
- and
- detecting the presence of an agent bound on the solid phase with a detection system that detects the bound tertiary detection agent;
- or
- (v) incubating the solid phase simultaneously with a primary detection agent, a secondary detection agent and a tertiary detection agent in solution;
- washing the solid phase to remove any unbound primary detection agent, secondary detection agent and tertiary detection agent;
- and
- detecting the presence of an agent bound on the solid phase with a detection system that detects the bound tertiary detection agent.
-24. (canceled)
Dependencies are inferred from the claim text.