Methods of detection using acousto-mechanical detection systems
OwnerSridhar V. Dasaratha, Samuel J. Gason +1inventors
InventorsSridhar V. Dasaratha, Samuel J. Gason, Paul N. Holt
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Abstract
Methods for detecting target biological analytes within sample material using acousto-mechanical energy generated by a sensor are disclosed. The acousto-mechanical energy may be provided using an acousto-mechanical sensor, e.g., a surface acoustic wave sensor such as, e.g., a shear horizontal surface acoustic wave sensor (e.g., a LSH-SAW sensor). The detection of the target biological analytes in sample material are enhanced by coupling of the target biological analyte (e.g., through the use of magnetic particles), application of a magnetic field to draw the target analyte to the sensor surface, and subsequent removal of the magnetic field before measuring detection.
Claims22
A method of detecting a target biological analyte, the method comprising:
- contacting sample material with magnetic particles, wherein a target biological analyte within the sample material interacts with the magnetic particles such that the target biological analyte is bound to the magnetic particle within the sample material;
- providing a system comprising an acousto-mechanical device comprising a detection surface with a capture agent located on the detection surface, wherein the capture agent is capable of selectively attaching the target biological analyte to the detection surface;
- contacting the detection surface of the acousto-mechanical device with the sample material;
- providing a magnetic field generator capable of providing a magnetic field proximate the detection surface that draws the target analyte with the attached magnetic particles to the sensor surface;
- selectively attaching the target biological analyte with the attached magnetic particles to the detection surface;
- disabling the magnetic field generator to substantially reduce the magnetic field proximate the detection surface;
- and
- operating the acousto-mechanical device to detect the attached target biological analyte while the detection surface is submersed in liquid.
A method of detecting a target biological analyte, the method comprising:
- fractionating target biological analyte located within sample material;
- contacting the fractionated target biological analyte with magnetic particles, wherein the fractionated target biological, analyte within the sample material interacts with the magnetic particles such that the fractionated target biological analyte is bound to the magnetic particle within the sample material;
- providing a system comprising a surface acoustic wave sensor comprising a detection surface with a capture agent located on the detection surface, wherein the capture agent is capable of selectively attaching the target biological analyte to the detection surface;
- contacting the detection surface of the surface acoustic wave device with the sample material;
- providing a magnetic field generator capable of providing a magnetic field proximate the detection surface that draws the target analyte attached to the magnetic particles to the sensor surface;
- selectively attaching the target biological analyte to the detection surface;
- removing the magnetic field generator a sufficient distance from the detection surface to substantially reduce the magnetic field proximate the detection surface;
- and
- operating the surface acoustic wave sensor to detect the attached fractionated target biological analyte while the detection surface is submersed in liquid.
Dependencies are inferred from the claim text.