Surface-enhanced raman scattering (sers) based nanoparticle composites
AssigneeLos Alamos National Security, LLC
InventorsKirk D. Rector, Kristy L. Nowak-Lovato
Life of this patent
Estimating the term…
Abstract
Nanoparticle composites and method of use thereof for simultaneously sensing and probing a biological system, comprising providing a nanoparticle composite comprising a nanoparticle comprising a core and a shell; a first ligand bound to the nanoparticle, said first ligand capable of sensing pH; a second ligand bound to the nanoparticle, said second ligand distinct from said first ligand and capable of binding to a target; and bringing the nanoparticle composite into contact with the biological system to produce a first and a second pH-dependent signal; and analyzing the first or the second signal by means of surface-enhanced Raman spectroscopy.
Claims20
A method of simultaneously sensing and probing a biological system comprising:
- a) providing a nanoparticle composite comprising:
- i. a nanoparticle comprising a core and a shell;
- ii. a first ligand bound to the nanoparticle, said first ligand capable of sensing pH;
- iii. a second ligand bound to the nanoparticle, said second ligand distinct from said first ligand and capable of binding to a target;
- b) bringing the nanoparticle composite into contact with the biological system to produce a first and a second pH-dependent signal;
- and
- c) analyzing the first and the second signal by means of surface-enhanced Raman spectroscopy.
The method of claim 9, wherein the pH is from about 4.0 to about 8.0.
A nanoparticle composite comprising:
- a) a nanoparticle comprising a core and a shell;
- b) a first ligand bound to the nanoparticle, said first ligand capable of sensing pH;
- and
- c) a second ligand bound to the nanoparticle, said second ligand distinct from said first ligand and capable of binding to a biological target.
A nanoparticle composite comprising:
- a) a nanoparticle comprising a noble metal;
- b) a first ligand bound to the nanoparticle, wherein the first ligand is 4-mercaptopyridine;
- c) a second ligand bound to the nanoparticle, wherein said second ligand is a 1,2-ε-dinitrophenol-L-lysine conjugate;
- and wherein the nanoparticle composite is suitable for use in analysis by surface-enhanced Raman spectroscopy.
Dependencies are inferred from the claim text.