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AssigneesStab Vida, InvestigaçãO E ServiçOs Em CiêNcias Bio, Universidade Nova De Lisboa
InventorsJosé Ricardo Ramos Franco Tavares, Pedro Miguel Ribeiro Viana Baptista, Gonçalo Maria Reimão Pinto De França Doria, Alcino Orfeu De Leoão Flores
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The present invention relates to a colorimetric method for the detection of specific nucleic acids sequences, including mutations or single nucleotide polymorphisms within nucleic acid sequences, through the aggregation of nanoparticles functionalized with modified oligonucleotides, induced by an increase of the medium's ionic strength. Another aspect of the present invention relates with the development of a kit based on the method of the present invention, allowing for a quick and easy detection of specific nucleic acids sequences, including mutations or single nucleotide polymorphisms within nucleic acid sequences.
A colorimetric method for the detection of specific nucleic acids sequences, including mutations or single nucleotide polymorphisms in nucleic acids sequences, comprising inducing aggregation of nanoparticles functionalized with modified oligonucleotides by increasing the ionic strength of the medium, also including a blank assay, a positive control assay, a negative control assay and one or more assays with the target nucleic acid sample, being that each assay includes a denaturation step, a hybridization step, a development step and a result recording step, sequentially by this order.
Dependencies are inferred from the claim text.