CpG island sequencing
Assignee454 Life Sciences Corporation
InventorsGisela Betzl, Bruno Frey, Epameinondas Fritzilas, Bernhard Horsthemke, Sven Rahmann, Michael Zeschnigk
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Abstract
The present invention is directed to a method for analyzing the methylation status of a genomic DNA sample, comprising the steps of (i) fragmenting said sample and enriching said sample for sequences comprising CpG islands, (ii) generating a single stranded DNA library, (iii) subjecting said sample to Bisulfite treatment, (iv) clonally amplifying individual members of said single stranded DNA library by means of emulsion PCR, and (v) sequencing said amplified clonally amplified single stranded DNA library.
Claims9
A method for analyzing the methylation status of a genomic DNA sample, comprising the steps of
- a) fragmenting said sample and enriching said sample for sequences comprising CpG islands,
- b) generating a single stranded DNA library,
- c) subjecting said sample to Bisulfite treatment,
- d) clonally amplifying individual members of said single stranded DNA library by means of emulsion PCR, and
- e) sequencing said clonally amplified single stranded DNA library.
Dependencies are inferred from the claim text.