Microvesicle-Based Compositions and Methods
AssigneeProxy Life Science Holdings, Inc.
InventorZbigniew Pietrzkowski
Life of this patent
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Abstract
Methods and compositions for diagnosis and/or analysis of a condition in a mammal are disclosed in which RNA from microvesicles is enriched and differentiated to so obtain a result that is indicative of the condition of tissue or organ from which the microvesicle originated. In especially preferred embodiments, the condition is a neoplastic disease of a human and can be identified and staged by differential analysis of one or more distinct RNAs, optionally together with identification and analysis of a non-RNA component of the microvesicle.
Claims18
A method of analyzing a biological sample of a mammal, comprising:
- obtaining a sample comprising a plurality of microvesicles that include a plurality of distinct RNA molecules from a living donor mammal;
- enriching and differentiating the microvesicles to produce at least one of (1) a primary result based on at least one of the plurality of distinct RNA molecules, (2) a secondary result based on at least two of the plurality of distinct RNA molecules, and (3) a ternary result based on a sub-segregated proxysome population and at least one of the plurality of distinct RNA molecules;
- and
- correlating at least one of the primary, secondary, and ternary results with a diagnosis or prognosis of a condition of the mammal.
A method of staging a mammalian neoplasm, comprising:
- obtaining a whole blood fraction that includes a plurality of microvesicles comprising a plurality of distinct RNA molecules;
- enriching and differentiating the microvesicles to produce at least one of (1) a primary result based on at least one of the plurality of distinct RNA molecules, (2) a secondary result based on at least two of the plurality of distinct RNA molecules, and (3) a ternary result based on a sub-segregated proxysome population and at least one of the plurality of distinct RNA molecules;
- and
- correlating at least one of the primary, secondary, and ternary results with a stage of the neoplasm in the mammal.
Dependencies are inferred from the claim text.