Detection of bacterium by utilizing dnaj gene and use thereof

-33. (canceled)

A process for detecting species of bacteria, in which two or more species of bacteria selected from among bacteria of the genera Staphylococcus, Streptococcus, Klebsiella, Escherichia, Vibrio, Bacillus, Neisseria, Campylobacter, Chlamydia, Chlamydophila and Mycoplasma, are subjects of detection, the process comprising:

• (a) a step of simultaneously contacting a test sample which may contain nucleic acids of the subject bacteria with two or more types of primer sets which hybridize with at least portions of DnaJ genes in the two or more subject bacterial species and perform amplification by PCR;
• and
• (b) a step of detecting amplified products amplified by the primer sets.

A nucleic acid molecule kit for detecting and identifying species of bacteria, in which two or more species of bacteria selected from among bacteria of the genera Staphylococcus, Streptococcus, Klebsiella, Escherichia, Vibrio, Bacillus, Neisseria, Campylobacter, Chlamydia, Chlamydophila and Mycoplasma are subjects of detection and identification,

• wherein the kit includes two or more primer sets which hybridize with at least portions of DnaJ genes in subject bacteria.

A method for amplifying two or more types of target nucleic acids, wherein,

• the method comprising a polymerase chain reaction step of performing a polymerase chain reaction using at least two types of first primer sets each having a tag sequence and a base sequence which selectively anneals to a portion of the target nucleic acid and at least two types of second primer sets each having a tag sequence which is substantially the same as the tag sequence of the respective first primer sets, wherein
• the polymerase chain reaction step simultaneously performs an amplification of the target nucleic acids with the first primer sets and an amplification of an amplified product obtained by the amplification with the first primer sets with the second primer sets all together, and
• the first primer sets are directed at, as a target nucleic acid thereof, a portion of a DnaJ gene of two or more species of bacteria selected from among bacteria of the genera Staphylococcus, Streptococcus, Klebsiella, Escherichia, Vibrio, Bacillus, Neisseria, Campylobacter, Chlamydia, Chlamydophila and Mycoplasma.