Colony Detection
AssigneeGenetix Limited
InventorsKerensa Klottrup, Christopher Mann, Aaron Figg, Julian Francis Burke
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Abstract
The present invention provides in one aspect a method for determining a production level of a polypeptide of interest by a cell or cell colony, comprising obtaining an image of one or more cells or cell colonies; and analyzing the image to detect a signal associated with the polypeptide of interest; wherein a signal level is determined for each cell or cell colony based on signal values from a predefined area surrounding the cell or cell colony, the signal level being indicative of the production level of the polypeptide of interest by the cell or cell colony.
Claims44
A method for determining a production level of a polypeptide of interest by a cell or cell colony, comprising:
- (a) obtaining an image of one or more cells or cell colonies;
- and
- (b) analyzing the image to detect a signal associated with the polypeptide of interest;
- wherein a signal level is determined for each cell or cell colony based on signal values from a predefined area surrounding the cell or cell colony, the signal level being indicative of the production level of the polypeptide of interest by the cell or cell colony.
A method for selecting a cell or cell colony which produces a polypeptide of interest, comprising:
- (a) determining a production level of the polypeptide of interest by a plurality of cells or cell colonies by a method as defined in any preceding claim;
- and
- (b) selecting one or more cells or cell colonies which produce the polypeptide of interest at an elevated level.
An automated imaging apparatus for determining a production level of a polypeptide of interest by a cell or cell colony, wherein the apparatus is configured to:
- (a) obtain an image of one or more cells or cell colonies;
- and
- (b) analyze the image to detect a signal associated with the polypeptide of interest;
- wherein the apparatus is configured to determine a signal level for each cell or cell colony based on signal values from a predefined area surrounding the cell or cell colony, the signal level being indicative of the production level of the polypeptide of interest by the cell or cell colony.
A computer program, residing on a computer-readable medium, for controlling automated image acquisition and analysis, the computer program comprising instructions for causing an apparatus to:
- (a) obtain an image of one or more cells or cell colonies;
- and
- (b) analyze the image to detect a signal associated with a polypeptide of interest, by determining a signal level for each cell or cell colony based on signal values from a predefined area surrounding the cell or cell colony, the signal level being indicative of the production level of the polypeptide of interest by the cell or cell colony.
Dependencies are inferred from the claim text.