Reconstruction and visualization of neuronal cell structures with bright-field mosaic microscopy

A method of reconstructing an image of a neuronal cell structure from a recorded image comprising an image stack of image layers, recorded with a bright field microscope, comprising:

• forming a corrected image by deconvoluting the recorded image, wherein deconvoluting comprises applying a linear deconvolution to the image stack on the basis of a point spread function of the bright field microscope, which point spread function is calculated on the basis of measured features of the bright field microscope, and
• extracting a cell structure image from the corrected image.

A reconstruction device that reconstructs an image of a neuronal cell structure from a recorded image comprising an image stack of image layers recorded with a bright field microscope, comprising:

• a deconvolution circuit that deconvolutes the recorded image and provides a corrected image, the deconvolution circuit being that which applies the deconvolution to the recorded image on the basis of measured features of a point spread function of the bright field microscope, and
• an extraction circuit adapted to extract a cell structure image from the corrected image.