High Throughput Screening, Purification and Recovery System for Large and Small Molecules

A method for screening and/or purifying one or a plurality of molecules comprising:

• providing a dipolar aprotic loading solvent or mixture of solvents having a polarity index greater than about 4.0;
• dissolving said sample in said loading solvent, wherein said sample comprises one or a plurality of molecules;
• loading said sample on a first chromatography column with said loading solvent;
• identifying a generic eluent system to elute said one or plurality of molecules in said sample on said first column;
• establishing a flow of said generic eluent system through said first chromatography column;
• and
• introducing said generic eluent system exiting said first chromatography column to a detector and detecting said one or plurality of molecules in said sample;
• optimizing said generic eluent system with respect to at least one of said plurality of molecules detected in said sample to provide an optimized eluent system relative to said generic eluent system;
• providing a second column for purifying and purifying said at least one of said plurality of molecules in said sample in said second column with said optimized eluent system.