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US 2010/0071259 A1Published application
Systems and methods for production of mixed fatty esters
AssigneeLS9, Inc.
InventorsZhihao Hu, Grace J. Lee, Douglas C. Cameron
Life of this patent
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Abstract
Disclosed herein are various embodiments regarding the production of mixed fatty esters. Disclosed herein are various embodiments regarding the use of mixed alcohol compositions for the production of fatty esters.
Claims41
A method of producing a fatty ester composition, comprising:
- identifying a desired fatty ester composition, wherein the fatty ester composition comprises at least a first fatty ester having the following formula:
- B1COOA1
- and a second fatty ester having the following formula:
- B2COOA2
- wherein B1 is a carbon chain that is at least 6 carbons in length, wherein B2 is a carbon chain that is at least 6 carbons in length, wherein A1 is an alkyl group of 1 to 5 carbons in length, wherein A2 is an alkyl group of 1 to 5 carbons in length, and wherein B1COOA1 is different from B2COOA2;
- selecting at least a first alcohol for an alcohol mixture, wherein the first alcohol and A1 have a same number of carbon atoms;
- selecting at least a second alcohol for the alcohol mixture, and wherein the second alcohol and A2 have a same number of carbon atoms;
- providing the alcohol mixture to a fatty ester production host;
- and
- converting the alcohols of the alcohol mixture to a fatty ester composition using the fatty ester production host.
7 more elements
A method of producing a fatty ester composition comprising:
- providing a mixture comprising methanol and at least one different alcohol having a different number of carbon atoms from methanol, wherein the mixture substantially lacks propanol;
- and
- producing fatty esters by providing the mixture to a fatty ester production host.
The method of claim 18, wherein the production host either lacks a nucleic acid sequence encoding for an acyl-CoA dehydrogenase enzyme or expresses an attenuated amount of an acyl-CoA dehydrogenase enzyme.
A method of producing at least two fatty esters, comprising:
- providing at least two different alcohols in a single mixture, the alcohols containing different numbers of carbon atoms;
- and
- adding the single mixture to a medium containing a fatty ester production host, whereby the fatty ester production host produces at least two different fatty esters.
A fatty ester composition, comprising:
- a production host comprising a nucleic acid sequence encoding for at least one or more of the following:
- a thioesterase enzyme, an acyl-CoA synthetase enzyme and an ester synthase enzyme;
- a first fatty ester having the following formula:
- B1COOA1
- a second fatty ester has the following formula:
- B2COOA2
- wherein B1 is a carbon chain that is at least 6 carbons in length, wherein B2 is a carbon chain that is at least 6 carbons in length, wherein A1 is an alkyl group of 1 to 5 carbons in length, wherein A2 is an alkyl group of 1 to 5 carbons in length, wherein B1 and B2 carbon chains have a number of carbon atoms independently selected from the group consisting of:
- 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, and 30.
5 more elements
A production system, comprising:
- a fatty ester production vessel;
- a first fatty ester having the following formula:
- B1COOA1
- a second fatty ester has the following formula:
- B2COOA2
- a third fatty ester has the following formula:
- B3COOA3
- wherein B1 is a carbon chain that is at least 6 carbons in length, B2 is a carbon chain that is at least 6 carbons in length, B3 is a carbon chain that is at least 6 carbons in length, A1 is an alkyl group of 1 to 5 carbons in length, A2 is an alkyl group of 1 to 5 carbons in length, and A3 is an alkyl group of 1 to 5 carbons in length.
5 more elements
A fatty ester composition comprising:
- a first fatty ester having the following formula:
- B1COOA1
- a second fatty ester has the following formula:
- B2COOA2
- wherein B1 is a carbon chain that is at least 6 carbons in length, wherein B2 is a carbon chain that is at least 6 carbons in length, wherein A1 is an alkyl group of 1 to 5 carbons in length, and A2 is an alkyl group of 1 to 5 carbons in length, and wherein A1 is different from A2.
2 more elements
A method of producing a fatty ester composition, comprising:
- selecting methanol as a first alcohol for an alcohol mixture;
- selecting a second alcohol for the alcohol mixture;
- providing the alcohol mixture to a fatty ester production host;
- and
- converting the alcohols of the alcohol mixture to a fatty ester composition using the fatty ester production host, whereby the presence of methanol in the alcohol mixture results in a fatty ester having the following formula:
- B1COOA1
- wherein B1 is a carbon chain that is at least 6 carbons in length and wherein A1 is an alkyl group of 1 carbon in length, wherein the fatty ester composition is biased to include more fatty esters having B1 selected from the group consisting of C16, C17, C18, and any combination thereof, in comparison to a method wherein the only alcohol is ethanol.
4 more elements
A method of producing a fatty ester composition, comprising:
- selecting ethanol as a first alcohol for an alcohol mixture;
- selecting a second alcohol for the alcohol mixture;
- providing the alcohol mixture to a fatty ester production host;
- and
- converting the alcohols of the alcohol mixture to a fatty ester composition using the fatty ester production host, whereby the presence of ethanol in the alcohol mixture results in a fatty ester having the following formula:
- B1COOA1
- wherein B1 is a carbon chain that is at least 6 carbons in length and wherein A1 is an alkyl group of 1 to 5 carbons in length, wherein the fatty ester composition is biased to include more fatty esters having B1 selected from the group consisting of C12, C13, C14, and any combination thereof, in comparison to a method wherein the only alcohol is methanol.
4 more elements
Dependencies are inferred from the claim text.