Modified chondroitin synthase polypeptide and crystal thereof
AssigneeSeikagaku Corporation
InventorsYoshimitsu Kakuta, Takuo Osawa, Nobuo Sugiura, Koji Kimata
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Abstract
Disclosed are: (A) a polypeptide consisting of the amino acid sequence of SEQ ID NO:2, or (B) a polypeptide comprising an amino acid sequence of SEQ ID NO:2 including deletion, substitution or addition of one or several amino acid residues and having chondroitin synthase activity; a nucleic acid encoding the polypeptide; a method for producing the polypeptide, comprising at least the steps of: (1) expressing the nucleic acid to produce the polypeptide; and (2) collecting the polypeptide produced in the step (1); and a crystal of the polypeptide. The crystal may be a monoclinic or tetragonal crystal.
Claims10
A polypeptide represented by the following (A) or (B):
- (A) a polypeptide consisting of the amino acid sequence of SEQ ID NO:
- 2;
- or
- (B) a polypeptide consisting of an amino acid sequence of SEQ ID NO:
- 2 including deletion, substitution, or addition of one or several amino acids and having chondroitin synthase activity.
A nucleic acid encoding the following polypeptide (A) or (B):
- (A) a polypeptide consisting of the amino acid sequence of SEQ ID NO:
- 2;
- or
- (B) a polypeptide consisting of an amino acid sequence of SEQ ID NO:
- 2 including deletion, substitution, or addition of one or several amino acids and having chondroitin synthase activity.
A nucleic acid represented by the following (a) or (b):
- (a) a DNA consisting of the nucleotide sequence of SEQ ID NO:
- 1;
- or
- (b) a DNA that hybridizes with a DNA consisting of the nucleotide sequence complementary to the DNA (a) under stringent conditions and has chondroitin synthase activity.
Dependencies are inferred from the claim text.