Nucleic acid extraction method

A method for extracting a nucleic acid, which comprises:

• (a) preparing a biomaterial containing a solution by a following step (i) or (ii):
• (i) a step in which a biomaterial containing a phosphate buffer solution or a Bis-Tris (N,N-bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane) buffer solution is prepared;
• or
• (ii) a step in which a buffer solution contained in a biomaterial is replaced with a Bis-Tris buffer solution;
• (b) dissolving the biomaterial by allowing the biomaterial to contact with a lysis solution, and eluting a nucleic acid contained in the biomaterial;
• (c) preparing a lysate solution by adding a water-soluble organic solvent to the nucleic acid-eluted solution obtained in the step (b);
• (d) allowing the nucleic acid contained in the lysate solution to be adsorbed by a solid material by allowing the lysate solution to contact with the solid material;
• (e) washing impurities remaining in the solid material, other than the nucleic acid to be extracted, and the lysis solution;
• and
• (f) desorbing the absorbed nucleic acid from the solid material by a recovering solution.