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US 2010/0063266 A1Published application
Method for Isolating Both Free and Protein Association DNA
AssigneeNorgen Biotek Corp.
InventorYousef Haj-Ahmad
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Abstract
A method is disclosed for isolating both free and protein-associated DNA from bodily fluids, such as urine, saliva, serum, tears, sweat, cerebral spinal fluid, and plasma. The method comprises as a first step concentrating and isolating both the free DNA and the proteins present in the bodily fluid. The proteins are then disgested in order to release the formerly protein-associated DNA from the isolated proteins. Lastly, the free and formerly protein-associated DNA can be isolated and purified.
Claims9
A method for isolating both free and protein-associated DNA from a bodily fluid comprising:
- a) first concentrating and isolating both free DNA and proteins present in the bodily fluid;
- b) digesting the proteins in order to release protein-associated DNA from the isolated proteins;
- and
- c) isolating the free and released total DNA.
1 more element
Dependencies are inferred from the claim text.