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US 2010/0063264 A1Published application
Nucleotide sequencing via repetitive single molecule hybridization
OwnerJoseph M. JACOBSONinventor
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Abstract
Methods of obtaining sequence information about target oligonucleotides by repetitive single molecule hybridization are disclosed. The methods include exposing a target oligonucleotide to one or more copies of a test oligonucleotide; measuring hybridization; dehybridizing the test oligonucleotide; and repeating until the information content from the hybridization trials equals or exceeds the information content of the target oligonucleotide.
Claims17
A method of synthesizing de novo a nucleic acid sequence, the method comprising the steps of:
- a) synthesizing a nucleic acid sequence de novo;
- b) obtaining information about the nucleic acid sequence by repetitive single molecule hybridization, thereby to detect an error in the nucleic acid sequence;
- and
- c) correcting the error in the nucleic acid sequence by site-directed mutagenesis, thereby synthesizing de novo the nucleic acid sequence.
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Dependencies are inferred from the claim text.