Back to results
US 2010/0063258 A1Published application
Fusion protein constructs
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsJames Robert Swartz, Junhao Yang, Alexei M. Voloshin
Life of this patent
Estimating the term…
Abstract
Polypeptide linkers with defined tertiary structures, usually of defined alpha helical structure, are used to join two domains in a fusion protein. In one embodiment of the invention, a method is provided for the cell-free synthesis of the fusion protein.
Claims14
A method for increased yield of a fusion protein, the method comprising:
- separating a first polypeptide of said fusion protein and a second polypeptide of said fusion protein with a linker of defined tertiary structure and at least about 45 amino acids in length;
- wherein the yield of full-length fusion protein is increased by at least 20%.
A fusion protein comprising:
- a first polypeptide and a second polypeptide, wherein said first polypeptide and said second polypeptide are joined by a linker of defined tertiary structure and at least about 45 amino acids in length.
Dependencies are inferred from the claim text.