Canola Protein Isolate Compositions
OwnerJames Logie, Radka Milanovainventors
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Abstract
A new canola protein isolate is provided along with a new canola protein. The new canola protein isolate is obtained from the supernatant from the production of a canola protein micellar mass and contains a predominance of 2S protein. The canola protein isolate derived from PMM contains a predominance of a 7S protein. Compositions of the canola protein isolate are provided.
Claims23
A canola protein isolate having a protein content of at least about 90 wt % on a dry weight basis and at a Kjeldahl nitrogen conversion of N×6.25 and which exhibits a protein profile which is:
- about 60 to about 95% wt % of 2S protein,
- about 5 to about 40 wt % of 7S protein, and
- 0 to about 5 wt % of 12S protein.
A canola protein isolate composition comprising:
- (1) a first canola protein isolate having a protein content of at least about 90 wt % on a dry weight basis and at a Kjeldahl nitrogen conversion of N×625 and which exhibits a protein profile which is:
- about 60 to about 98 wt % of 7S protein,
- about 1 to about 15 wt % of 12S protein,
- 0 to about 25 wt % of 2S protein, and
- (2) a second canola protein isolate having a protein content of at least about 90 wt % on a dry weight basis and a Kjeldahl nitrogen conversion of N×6.25 and which exhibits a protein profile which is;
- about 60 to about 95 wt % of 2S protein
- about 5 to about 40 wt % of 7S protein
- 0 to about 5 wt % of 12S protein.
An isolated and purified 7S protein of canola.
A composition, comprising a mixture of an isolated and purified 7S protein of canola protein and at least one additional isolated and purified canola protein extracted from the group consisting of the 2S and 12S canola proteins.
A method of isolating and purifying an individual canola protein from a canola protein isolate containing a mixture of at least two different canola proteins selected from the group consisting of the 2S, 7S and 12S proteins, which comprises:
- providing said canola protein isolate having a protein contents of at least about 90 wt % on a dry weight basis and at a Kjeldahl nitrogen conversion of N×6.25,
- solubilizing the canola protein isolate,
- subjecting the resulting protein solution to preparative high pressure liquid chromatography or equivalent procedure separating proteins on the basis of molecular size,
- collecting fractions of eluant containing the individual selected canola protein,
- subjecting the selected fractions to ultrafiltration to reduce the volume of eluant,
- dialyzing the concentrated eluant to remove solubilizing material, and drying the dialyzate to recover the individual canola protein.
Dependencies are inferred from the claim text.