Psod expression units
AssigneePaik Kwang Industrial Co., Ltd.
InventorsBurkhard KROGER, Oskar ZELDER, Corinna KLOPPROGGE, Hartwig SCHRODER, Stefan HAEFNER
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Abstract
The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.
Claims9
An isolated nucleic acid molecule having promoter activity, selected from the group consisting of
- A) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:1;
- B) a nucleic acid molecule comprising a nucleotide sequence of at least 90% identity to the nucleotide sequence of SEQ ID NO:1;
- C) a nucleic acid molecule which hybridizes with the complement of the nucleotide sequence of SEQ ID NO:1;
- and
- D) a nucleic acid molecule comprising a fragment of the nucleic acid molecule of (A), (B) or (C),
- wherein the molecule has promoter activity;
- wherein the nucleic acid molecule does not consist of SEQ ID NO:1.
A method for altering or causing the transcription rate of genes in microorganisms compared with the wild type by
- a) altering the specific promoter activity in the microorganism of endogenous nucleic acids having promoter activity, which regulate the transcription of endogenous genes, compared with the wild type or
- b) regulating the transcription of genes in the microorganism by nucleic acids having promoter activity or by nucleic acids having promoter activity with altered specific promoter activity according to embodiment a), where the genes are heterologous in relation to the nucleic acids having promoter activity.
Dependencies are inferred from the claim text.