Methods And Compositions For Increasing Replication Capacity Of An Influenza Virus
AssigneeMedImmune, LLC
InventorsHong Jin, Bin Lu
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Abstract
In certain aspects, the present invention provides methods for increasing the replication capacity of influenza viruses in hens' eggs and/or cell culture, recombinant and/or reassortant influenza viruses with increased replication capacity, and immunogenic and vaccine compositions comprising such recombinant and/or reassortant influenza viruses. In other aspects, the invention further provides nucleic acids encoding influenza genes associated with increased replication capacity, expression vectors comprising the nucleic acids of the invention, methods for making influenza viruses with increased replication capacity, and kits useful for practice of the methods.
Claims25
A method for increasing the replication capacity of an influenza virus, comprising altering an amino acid at a position corresponding to at least one of position 95, 143, 144 or 218 of the M1 protein of influenza strain MDV-A, thereby increasing the replication capacity of the influenza virus, with the proviso that the amino acid corresponding to position 218 is not altered to be a threonine (T).
A method for increasing the replication capacity of an influenza virus, comprising altering amino acids at positions corresponding to positions 95, 143, 144 and 218 of the M1 protein of influenza strain MDV-A, thereby increasing the replication capacity of the influenza virus.
19-21. (canceled)
23-51. (canceled)
An expression vector comprising a nucleic acid sequence operably linked to a pol I promoter, wherein the nucleic acid sequence encodes an influenza M1 protein comprising lysine (K) at position 95, alanine (A) at position 143, phenylalanine (F) at position 144, or isoleucine (I) at position 218, wherein the positions correspond to the M1 protein of influenza strain MDV-A, with the proviso that the sequence does not encode a wild-type M1 protein of influenza strain A/PR/8/34.
55-56. (canceled)
Dependencies are inferred from the claim text.