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AssigneeToyo Seikan Kaisha, Ltd.
InventorsSatoshi Tanaka, Yoichi Ishizaki, Ryo Suenaga, Yoriko Tokita, Masahito Kogure
Estimating the term…
In a cell culture in a sealed cell culture container, gradual deterioration of culture environment is prevented. A cell culture method in which cell culture is conducted by adding a fresh culture medium to a culture container in which cells and a culture medium are enclosed, which includes the steps of adjusting the pH of said culture medium to be added to be higher than the pH which is optimum for culture of the cells, and/or adjusting the partial pressure of dissolved carbon dioxide of the culture medium to be added to be lower than the pressure which is optimum for culture of the cells, and/or adjusting the partial pressure of dissolved oxygen of the culture medium to be added to be higher than the pressure which is optimum for culture of the cells; and mixing the adjusted culture medium and the culture medium in said culture container to adjust at least any of the pH, the partial pressure of dissolved carbon dioxide and the partial pressure of dissolved oxygen to be optimum for culture of said cells, thereby to culture the cells in the culture container.
A cell culture method in which a fresh culture medium is added to a culture container where cells and a culture medium are enclosed to culture said cells, comprising the steps of:
A cell culture system in which a fresh culture medium is added to a culture container in which cells and a culture medium are enclosed, wherein said cell culture system is provided with a culture medium adjusting apparatus which adjusts the pH and the amount of a dissolved gas of said culture medium to be added, a culture container in which a culture medium and cells are enclosed, and a tube which supplies said culture medium to be added from said culture medium adjustment apparatus to said culture container.
A culture medium adjustment apparatus in which a fresh culture medium is added to a culture container in which cells and a culture medium are enclosed, wherein the pH of said culture medium to be added is adjusted to be higher than the pH which is optimum for culture of said cells, and/or the partial pressure of dissolved carbon dioxide of said culture medium to be added is adjusted to be lower than the pressure which is optimum for culture of said cells, and/or the partial pressure of dissolved oxygen of said culture medium to be added is adjusted to be higher than the pressure which is optimum for culture of said cells.
Dependencies are inferred from the claim text.