Induction, propagation and isolation of liver progenitor cells
AssigneeUniversity of Southern California
InventorsMartin F. Pera, Tracy L. Zinberg, Kouichi Hasegawa
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Abstract
The present invention relates to methods of induction and isolation of progenitor cells from stem cell cultures, specifically liver progenitor cells from human embryonic stem cell cultures. In one embodiment, the present invention provides a method of inducing hepatocyte-like progenitor cells by placing a quantity of human embryonic stem cells in a medium supplemented with an inhibitor of the MAPK/MEK/ERK signaling pathway, FGFR, GSK3 and/or BMP.
Claims17
A method of inducing and/or propagating a quantity of progenitor cells, comprising:
- providing a quantity of human stem cells;
- and
- placing the quantity of human stem cells in a medium supplemented with one or more of an inhibitor of the mitogen-activated protein kinase (MAPK)/map-erk kinase (MEK)/extracellular signal-regulated kinase (ERK) signaling pathway, a fibroblast growth factor receptor (FGFR) inhibitor, and a glycogen synthase kinase 3 (GSK3) inhibitor.
A composition comprising an inhibitor of the MAPK/MEK/ERK signaling pathway, an FGFR inhibitor, a GSK3 inhibitor, and a BMP inhibitor.
A composition comprising activin, BMP, epidermal growth factor (EGF), heregulin, fibroblast growth factor 1 (FGF-1), insulin growth factor (IGF), vascular endothelial growth factor (VEGF), angiopoietin, and transforming growth factor beta (TGF beta).
Dependencies are inferred from the claim text.