Optimized messenger rna
OwnerRichard F. Selden, Allan M. Miller +1inventors
InventorsRichard F. Selden, Allan M. Miller, Douglas A. Treco
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Abstract
The present invention is directed to a synthetic nucleic acid sequence which encodes a protein wherein at least one non-common codon or less-common codon is replaced by a common codon. The synthetic nucleic acid sequence can include a continuous stretch of at least 90 codons all of which are common codons.
Claims5
A synthetic nucleic acid sequence which encodes a protein wherein at least one non-common codon or less-common codon has been replaced by a common codon, and wherein the synthetic nucleic acid sequence comprises a continuous stretch of at least 90 codons all of which are common codons, or wherein the synthetic nucleic acid sequence comprises a continuous stretch of common codons, which continuous stretch includes at least 33% or more of the codons in the synthetic nucleic acid sequence, or wherein at least 94% or more of the codons in the sequence encoding the protein are common codons and the synthetic nucleic acid sequence encodes a protein of at least about 90 amino acids in length, and wherein the protein is selected from the group consisting of:
- blood clotting factor V, blood clotting factor VII, blood clotting factor X, blood clotting factor XIII;
- an interleukin;
- erythropoietin (EPO);
- calcitonin;
- growth hormone;
- insulin;
- insulinotropin;
- an insulin-like growth factor;
- parathyroid hormone;
- β-interferon;
- γ-interferon;
- a nerve growth factor;
- FSFβ;
- tumor necrosis factor;
- glucagon;
- bone growth factor-2;
- bone growth factor-7 TSH-β;
- CSF-granulocyte;
- CSF-macrophage;
- CSF-granulocyte/macrophage;
- an immunoglobulin;
- a catalytic antibody;
- protein kinase C;
- glucocerebrosidase;
- superoxide dismutase;
- tissue plasminogen activator;
- urokinase;
- antithrombin III;
- DNAse;
- tyrosine hydroxylase;
- apolipoprotein E;
- apolipoprotein A-I;
- a globin;
- low density lipoprotein receptor;
- IL-2 receptor;
- an IL-2 antagonist;
- alpha-1 antitrypsin;
- soluble CD4;
- a protein encoded by a virus;
- an antigen;
- a protein which does not occur in nature;
- glucogen-like peptide-1 (GLP-1);
- β-glucoceramidase;
- α-iduronidase;
- α-L-iduronidase;
- glucosamine-N-sulfatase;
- alpha-N-acetylglucosaminidase;
- acetylcoenzyme A:α-glucosmamide-N-acetyltransferase;
- N-acetylglucosamine-6-sulfatase;
- β-galactosidase;
- N-acetylgalactosamine-6-sulfatase;
- and β-glucuronidase.
Dependencies are inferred from the claim text.