Concentrating White Blood Cells for DNA Extraction from a Leukodepleted Blood Sample

Abstract

Reagents and a method for the pre-concentration of WBCs from leukodepleted blood segment samples are described. The reagent comprises: a lytic reagent, for example, saponin in phosphate buffered saline(PBS), wherein the amount of saponin is in the range of from about 1% to about 10% percent (w/w). The method comprises: contacting a specific volume the leukoreduced blood segment sample with a specific volume of the lytic regent; mixing the two so as to selectively lyse RBCs in the leukoreduced whole blood segment sample; subjecting the mixture to centrifugation, so as to separate the mixture into a WBC rich phase and a lysed-RBC phase; discarding the supernatant containing the lysed RBC phase; and resuspending the WBC rich phase in a specific volume of PBS.

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