Recombinant n-glycosylated proteins from procaryotic cells
AssigneeEidgenossische Technische Hochschule Zurich
InventorsMarkus Aebi, Machael Kowarik, Umesh Ahuja
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Abstract
The present invention relates to recombinant N-glycosylated proteins, comprising one or more introduced N-glycosylated optimized amino acid sequence(s), nucleic acids encoding these proteins as well as corresponding vectors and host cells. In addition, the present invention is directed to the use of said proteins, nucleic acids, vectors and host cells for preparing medicaments. Furthermore, the present invention provides methods for producing said proteins.
Claims33
A recombinant N-glycosylated protein, comprising one or more of the following N-glycosylated optimized amino acid consensus sequence(s):
- D/E-X-N-Z-S/T,
- wherein X and Z may be any natural amino acid except Pro, and wherein at least one of said N-glycosylated optimized amino acid consensus sequence(s) is introduced.
A method for producing N-linked glycosylated proteins, comprising:
- a) providing a recombinant organism, preferably a prokaryotic organism, comprising nucleic acids coding for
- i) a functional pgl operon from Campylobacter spp., preferably C. jejuni, and
- ii) at least one recombinant target protein comprising one or more of the following N-glycosylated optimized amino acid consensus sequence(s):
- D/E-X-N-Z-S/T,
- wherein X and Z may be any natural amino acid except Pro, and wherein at least one of said N-glycosylated optimized amino acid consensus sequence(s) is introduced, and
- b) culturing the recombinant organism in a manner suitable for the production and N-glycosylation of the target protein(s).
Dependencies are inferred from the claim text.