Modulating plant tocopherol levels
OwnerSteven Craig Bobzin, Boris Jankowski +3inventors
InventorsSteven Craig Bobzin, Boris Jankowski, Amr Saad Ragab, Joon-Hyun Park, Jennifer E. Van Fleet
Life of this patent
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Abstract
Plants and plant cells having modulated levels of tocopherols and/or tocotrienols are described herein. Materials and methods for making plants and plant cells with modulated levels of tocopherols and/or tocotrienols are also described.
Claims49
A method of producing a plant tissue, said method comprising growing a plant cell comprising an exogenous nucleic acid comprising a nucleotide sequence encoding a polypeptide having 80% or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:2-15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NOs:25-30, SEQ ID NOs:32-46, SEQ ID NOs:48-50, SEQ ID NOs:52-55, SEQ ID NOs:57-62, SEQ ID NOs:64-69, SEQ ID NOs:71-73, SEQ ID NO:75, SEQ ID NOs:77-86, SEQ ID NOs:88-91, SEQ ID NOs:93-95, SEQ ID NOs:97-99, SEQ ID NOs:101-102, and the consensus sequences set forth in FIGS. 7-13, wherein said tissue has a difference in the level of one or both of a tocopherol and a tocotrienol as compared to the corresponding level in tissue of a control plant that does not comprise said nucleic acid.
A method of producing a plant tissue, said method comprising growing a plant cell comprising at least two nucleotide sequences, wherein each nucleotide sequence encodes a polypeptide having 80% or greater sequence identity to an amino acid sequence selected from the group consisting of:
- (a) SEQ ID NOs:2-15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, and the consensus sequence set forth in FIG. 7;
- (b) SEQ ID NOs:25-30 and the consensus sequence set forth in FIG. 8;
- (c) SEQ ID NOs:32-46 and the consensus sequence set forth in FIG. 9;
- (d) SEQ ID NOs:48-50, SEQ ID NOs:52-55, SEQ ID NOs:57-62, and the consensus sequence set forth in FIG. 10;
- (e) SEQ ID NOs:64-69, SEQ ID NOs:71-73, SEQ ID NO:75, and the consensus sequence set forth in FIG. 11;
- (f) SEQ ID NOs:77-86 and the consensus sequence set forth in FIG. 12;
- and
- (g) SEQ ID NOs:88-91, SEQ ID NOs:93-95, SEQ ID NOs:97-99, SEQ ID NOs:101-102, and the consensus sequence set forth in FIG. 13;
- wherein each of said at least two nucleotide sequences is from a different one of (a), (b), (c), (d), (e), (f), or (g);
- and wherein said tissue has a difference in the level of one or both of a tocopherol and a tocotrienol as compared to the corresponding level in tissue of a control plant that does not comprise said at least two nucleotide sequences.
7-12. (canceled)
An isolated nucleic acid molecule comprising a nucleotide sequence having 95% or greater sequence identity to the nucleotide sequence set forth in SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:51, SEQ ID NO:56, SEQ ID NO:70, SEQ ID NO:74, SEQ ID NO:92, SEQ ID NO:96, or SEQ ID NO:100.
An isolated nucleic acid comprising a nucleotide sequence encoding a polypeptide having 80% or greater sequence identity to the amino acid sequence set forth in SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:52, SEQ ID NO:57, SEQ ID NO:71, SEQ ID NO:75, SEQ ID NO:93, SEQ ID NO:97, or SEQ ID NO:101.
39-58. (canceled)
A plant comprising an exogenous nucleic acid comprising a nucleotide sequence encoding a polypeptide having 80% or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:2-15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NOs:25-30, SEQ ID NOs:32-46, SEQ ID NOs:48-50, SEQ ID NOs:52-55, SEQ ID NOs:57-62, SEQ ID NOs:64-69, SEQ ID NOs:71-73, SEQ ID NO:75, SEQ ID NOs:77-86, SEQ ID NOs:88-91, SEQ ID NOs:93-95, SEQ ID NOs:97-99, SEQ ID NOs:101-102, and the consensus sequences set forth in FIGS. 7-13, wherein one or more tissues of said plant have a difference in the level of one or both of a tocopherol and a tocotrienol as compared to the corresponding level in tissue of a control plant that does not comprise said nucleic acid.
A plant comprising at least two nucleotide sequences, wherein each nucleotide sequence encodes a polypeptide having 80% or greater sequence identity to an amino acid sequence selected from the group consisting of:
- (a) SEQ ID NOs:2-15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, and the consensus sequence set forth in FIG. 7;
- (b) SEQ ID NOs:25-30 and the consensus sequence set forth in FIG. 8;
- (c) SEQ ID NOs:32-46 and the consensus sequence set forth in FIG. 9;
- (d) SEQ ID NOs:48-50, SEQ ID NOs:52-55, SEQ ID NOs:57-62, and the consensus sequence set forth in FIG. 10;
- (e) SEQ ID NOs:64-69, SEQ ID NOs:71-73, SEQ ID NO:75, and the consensus sequence set forth in FIG. 11;
- (f) SEQ ID NOs:77-86 and the consensus sequence set forth in FIG. 12;
- and
- (g) SEQ ID NOs:88-91, SEQ ID NOs:93-95, SEQ ID NOs:97-99, SEQ ID NOs:101-102, and the consensus sequence set forth in FIG. 13;
- wherein each of said at least two nucleotide sequences is from a different one of (a), (b), (c), (d), (e), (f), or (g);
- and wherein one or more tissues of said plant have a difference in the level of one or both of a tocopherol and a tocotrienol as compared to the corresponding level in tissue of a control plant that does not comprise said at least two nucleotide sequences.
A method of making a plant, comprising:
- a) obtaining a plurality of plants transformed with an exogenous nucleic acid, said exogenous nucleic acid comprising a nucleotide sequence encoding a polypeptide having 80% or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:2-15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NOs:25-30, SEQ ID NOs:32-46, SEQ ID NOs:48-50, SEQ ID NOs:52-55, SEQ ID NOs:57-62, SEQ ID NOs:64-69, SEQ ID NOs:71-73, SEQ ID NO:75, SEQ ID NOs:77-86, SEQ ID NOs:88-91, SEQ ID NOs:93-95, SEQ ID NOs:97-99, SEQ ID NOs:101-102, and the consensus sequences set forth in FIGS. 7-13, said nucleotide sequence operably linked to a regulatory region;
- and
- b) selecting from said plurality of plants at least one plant in which one or more tissues of said plant have a difference in the level of one or both of a tocopherol and a tocotrienol as compared to the corresponding level in tissue of a control plant that does not comprise said nucleic acid.
A method of enhancing the nutritional value of a plant, said method comprising growing a plant comprising an exogenous nucleic acid comprising a nucleotide sequence encoding a polypeptide having 80% or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:2-15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NOs:25-30, SEQ ID NOs:32-46, SEQ ID NOs:48-50, SEQ ID NOs:52-55, SEQ ID NOs:57-62, SEQ ID NOs:64-69, SEQ ID NOs:71-73, SEQ ID NO:75, SEQ ID NOs:77-86, SEQ ID NOs:88-91, SEQ ID NOs:93-95, SEQ ID NOs:97-99, SEQ ID NOs:101-102, and the consensus sequences set forth in FIGS. 7-13, wherein a tissue of said plant has an increased level of one or both of a tocopherol and a tocotrienol as compared to the corresponding level in tissue of a control plant that does not comprise said nucleic acid.
A method of enhancing the nutritional value of a plant, said method comprising growing a plant comprising at least two nucleotide sequences, wherein each nucleotide sequence encodes a polypeptide having 80% or greater sequence identity to an amino acid sequence selected from the group consisting of:
- (a) SEQ ID NOs:2-15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, and the consensus sequence set forth in FIG. 7;
- (b) SEQ ID NOs:25-30 and the consensus sequence set forth in FIG. 8;
- (c) SEQ ID NOs:32-46 and the consensus sequence set forth in FIG. 9;
- (d) SEQ ID NOs:48-50, SEQ ID NOs:52-55, SEQ ID NOs:57-62, and the consensus sequence set forth in FIG. 10;
- (e) SEQ ID NOs:64-69, SEQ ID NOs:71-73, SEQ ID NO:75, and the consensus sequence set forth in FIG. 11;
- (f) SEQ ID NOs:77-86 and the consensus sequence set forth in FIG. 12;
- and
- (g) SEQ ID NOs:88-91, SEQ ID NOs:93-95, SEQ ID NOs:97-99, SEQ ID NOs:101-102, and the consensus sequence set forth in FIG. 13;
- wherein each of said at least two nucleotide sequences is from a different one of (a), (b), (c), (d), (e), (f), or (g);
- and wherein a tissue of said plant has an increased level of one or both of a tocopherol and a tocotrienol as compared to the corresponding level in tissue of a control plant that does not comprise said at least two nucleotide sequences.
Dependencies are inferred from the claim text.