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AssigneeNational University of Ireland, Galway
InventorsTyrone Villalard Bowes, Udo Greiser, William James Johnathan Finlay, Timothy O'Brien, Frank Barry
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Abstract Markers, antibodies and recombinant scFvs for Mesenchymal Stem Cell sub-populations and osteoclasts. The present invention relates to specific epitopes of surface membrane bound glycoproteins expressed by mesenchymal stem cells and pre-osteoclasts and relates to antibodies such as monoclonal antibodies and recombinant scFv or fragments thereof, raised to the particular epitope and their use in identifying, isolating, and characterization of mesenchymal stem cell sub-populations such as that termed “Stromal Progenitor Cells” (SPCs) in bone marrow and identifying, isolating, and characterization of pre-osteoclasts in peripheral blood. By binding to a specific epitope on the cell surface, limbin/EVC-2 detection and separation by conventional cell sorting methodologies are facilitated.
A polypeptide comprising an amino acid sequence comprising SEQ ID NO:
A nucleic acid sequence comprising SEQ ID No. 2 or a sequence substantially homologous thereto.
An antibody comprising the amino-acid sequence SEQ ID No. 3 or the amino-acid sequence encoded by SEQ ID No. 4.
A nucleic acid molecule comprising a sequence selected from the group comprising SEQ ID NO:4, SEQ ID NO:
A polypeptide comprising a sequence selected from the group comprising SEQ ID No. 3, SEQ ID NO. 5, SEQ ID NO. 7, SEQ ID NO. 9 or a sequence substantially homologous thereto.
A nucleic acid sequence selected from the group consisting of SEQ ID NOs. 11 to 18.
Use of any of SEQ ID Nos 1-18 in a method of cancer diagnosis.
A humanised antibody which is a humanised version of SEQ ID NO. 3, 5, 7 or 9.
Use of any of SEQ ID Nos 1-18 in a diagnostic method for osteoporosis.
A polypeptide, nucleic acid molecule, antibody, vector expression system, cell or cell population, pharmaceutical composition, use, medical device, gene delivery system or method substantially as herein described with reference to the accompanying figures, tables, sequences and appendices.
Dependencies are inferred from the claim text.