Hemagglutinin Polypeptides, and Reagents and Methods Relating Thereto
AssigneeMassachusetts Institute of Technology
InventorsRam Sasisekharan, Karthik Viswanathan, Aarthi Chandrasekaran, Rahul Raman, Aravind Srinivasan, S. Raguram, Viswanathan Sasisekharan
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Abstract
The present invention provides a system for analyzing interactions between glycans and interaction partners that bind to them. The present invention also provides HA polypeptides that bind to umbrella-topology glycans, and reagents and methods relating thereto.
Claims38
1-35. (canceled)
A pharmaceutical composition comprising:
- an agent that competes a glycan-HA polypeptide interaction between umbrella-topology glycans and an HA polypeptide.
A method of identifying agents that compete with a glycan-HA polypeptide interaction by:
- providing a collection of test agents;
- contacting the test agents with at least one umbrella-topology glycan and at least one HA polypeptide that binds to the umbrella-topology glycan;
- and
- determining that observed binding between the at least one umbrella-topology glycan and the at least one HA polypeptide is reduced when the agent is present as compared with when it is absent.
A method of identifying agents with high affinity for umbrella-topology glycans by:
- providing a collection of test agents;
- contacting the test agents with at least one umbrella-topology glycan;
- and
- determining that observed binding between the at least one umbrella-topology glycan and the test agent occurs with high affinity.
A method of detecting HA polypeptides in a sample, wherein the HA polypeptides bind to umbrella topology glycans.
A method of evaluating a test agent with high affinity for umbrella-topology glycans comprising,
- determining the ability of the agent to bind to an HA having an umbrella topology glycan,
- thereby evaluating the agent.
A device containing an agent with high affinity for umbrella-topology glycans and configured to administer a dose of an agent to the respiratory tract of a subject.
A glycan array comprising glycan structures of at least about 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90% 95%, or more of glycans found on HA receptors in human upper respiratory tract tissues.
An antibody that binds to an umbrella-topology glycan.
Dependencies are inferred from the claim text.