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US 2010/0061618 A1Published application
Methods of chromogen separation-based image analysis
AssigneeTriPath Imaging, Inc.
InventorsRaphaël Marcelpoil, Ryan Williams, Cédrick Orny
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Abstract
Methods for chromogen separation-based image analysis are provided, with such methods being directed to quantitative video-microscopy techniques in cellular biology and pathology applications.
Claims4
A method of selecting a region of interest on a slide, the region being positively contrasted from a surrounding thereof in a marker-only image corresponding to an RGB image of the sample, the positively contrasted region including at least one of a relatively larger nuclei and a relatively higher cell density than the surrounding, comprising:
- applying a low pass filter to a marker-only image of a sample, the marker-only image being obtained through chromogen separation of the RGB image of the sample;
- determining a marker-only histogram of pixels in the marker-only image;
- and
- binarizing the marker-only image according to a threshold in the marker-only histogram so as to form a mask for discriminating between negative and positive regions of the sample.
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