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AssigneesKanagawa Academy of Science and Technology, Institute of Microchemical Technology Co., Ltd., The University of Tokyo
InventorsMasayo Yamauchi, Akihide Hibara, Takehiko Kitamori, Kazuma Mawatari, Manabu Tokeshi
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An objective of the present invention is to provide a circular dichroism thermal lens microscope apparatus capable of identifying and quantifying optically active samples in ultra-trace amounts, and which has a higher sensitivity than conventional apparatuses. The objective is achieved by a circular dichroism thermal lens microscope apparatus which beams excitation light and detection light into an optical microscope, where the detection light enters a thermal lens formed by irradiating a sample with the excitation light, and a substance in a sample is detected by determining the diffusion of the detection light by the thermal lens, and where the excitation light is modulated by a phase-modulation element, so as to identify or quantify an optical isomer.
A circular dichroism thermal lens microscope apparatus, which comprises separate oscillation means for emitting excitation light and for emitting detection light of different wavelengths which wavelengths are introduced into a sample via an objective lens, wherein changes in the amount of the transmitted detection light caused by a thermal lens formed in the sample using said excitation light is determined as circular dichroism thermal lens signals by an optical detector, and wherein the apparatus further comprises a phase-modulation means, which is capable of modulating said excitation light into right- and left-circularly polarized light, which phase-modulation means is placed on an excitation light path between said oscillation means and the sample, and wherein the optical detector identifies and quantifies an optical isomer by determining the difference in thermal lens signals (also referred to as circular dichroism thermal lens signals) caused by irradiating the sample with said right- and left-circularly polarized light.
A circular dichroism thermal lens microscope apparatus, which comprises separate oscillation means for emitting excitation light and for emitting detection light of different wavelengths, which wavelengths are introduced into the sample via an objective lens for focusing said excitation light in the sample, and which apparatus determines the intensity of the detection light passing through the sample, wherein the apparatus further comprises a phase-modulation means, which is capable of selectively modulating only said excitation light into right- and left-circularly polarized light, which phase-modulation means is placed on an excitation light path between said oscillation means and the sample, and a synchronous detection means capable of detecting the intensity of the detection light passing through the sample by synchronizing with the modulation of said phase-modulation means, and wherein the synchronous detection means determines at least the phase difference of a synchronized component by extracting the component synchronizing with the phase-modulation from the intensity of the detection light passing through the sample.
A circular dichroism thermal lens microscope apparatus, which comprises a first oscillator which emits excitation light and a second oscillator which emits detection light, wherein the excitation light and the detection light are of different wavelengths, which wavelengths are introduced into a sample via an objective lens, wherein changes in the amount of the transmitted detection light caused by a thermal lens formed in the sample using said excitation light is determined as circular dichroism thermal lens signals by an optical detector, and wherein the apparatus further comprises a phase-modulator, which is capable of modulating said excitation light into right- and left-circularly polarized light, which phase-modulator is placed on an excitation light path between said excitation light emitting oscillator and the sample, and wherein the optical detector identifies and quantifies an optical isomer by determining the difference in circular dichroism thermal lens signals caused by irradiating the sample with said right- and left-circularly polarized light.
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