A gas supply assembly, comprising a gas supply manifold arranged to receive discharged gas from a pressure-regulated gas source vessel for delivery to a gas-using zone, wherein the gas supply manifold is isolatable from the pressure-regulated gas source vessel when a predetermined pressure occurrence is detected in the gas supply manifold, an isolation valve actuatable to isolate the gas supply manifold from the pressure-regulated gas source vessel upon said predetermined pressure occurrence, and a pressure sensing element arranged to sense said predetermined pressure occurrence in the gas supply manifold, with the isolation valve being arranged for actuation to isolate the gas supply manifold from the pressure-regulated gas source vessel in response to sensing of said predetermined pressure occurrence by the pressure sensing element, wherein the pressure-regulated gas source vessel comprises an interior volume, and a gas regulator or a series-arranged assembly of gas regulators is in said interior volume to regulate gas dispensing from the vessel.